Manufacturer: Cold Spring Harbor Laboratory Press Availability: Usually ships in 24 hours List Price:$259.00 Our Price:$259.00 Authors: Joseph Sambrook David W. Russell Description:

At the beginning of each chapter, the authors give an introduction to the protocols and this is of an enormous help to those readers with only rudimentary acquaintance with the laboratory procedures. Typically, this introduction contains an historical summary of the procedures as they were developed or discovered. One can only marvel at the ingenuity of the discoverers of these techniques. These introductions are fairly straightforward to read, even for those that are not experts in biochemistry.

At the end of each chapter, the authors include an "information panel" that gives a more in-depth view of the biochemistry or genetics behind the procedures. These are summaries and are highly specialized, and are again meant for experienced readers. A very lengthy list of references is also included at the end of each chapter.

Becuase of the size of this collection, space here does not permit a detailed review, so I will list some of the areas that I thought were particularly interesting or well-written (these coming from the introduction or the information panels only): 1. The DNA synthesis at the colE1 replicon and the interaction between RNAI and RNAII. 2. The discussion of electroporation and the physics behind this technique to introduce DNA into eukaryotic cells. 3. The discussion on the discovery of bacteriophage lambda. 4. The discussion (with diagram), of the assembly pathway of bacteriophage lambda. 5. The summary of the early analysis of DNA using electrophoresis and the different pulsed-field configurations used. 6. The anecdote on the discovery of the polymerase chain reaction. 7. The short discussion on computer-assisted design of oligonucleotide primers. 8. The discussion of oligonucleotide synthesis. 9. The flowchart detailing the preparing and screening of a cDNA library. 10. The history of the development of the methods to synthesize and clone cDNAs. 11. The detailed discussion of the molecular cloning of double-stranded cDNA. 12. The discussion on the methods to validate clones of cDNA. 13. The discussion on magnetic beads for affinity purification. 14. The discussion on the history of DNA sequencing and the different techniques to accomplish it, particularly the information panel on automated DNA sequencing. 15. The discussion of the different types of mutagenesis and the different methods for accomplishing it. 18. The fascinating discussion of how to introduce cloned genes into mammalian cells. 19. The discussion on the steps involved in DNA footprinting. 20 The discussion on green flourescent protein and its use as a fusion tag. 21. The discussion on the use of surface plasmon resonance spectroscopy.


A must for molecular biologists
I like this book, however some fields better are covered in current protocols in molecular biology, and vice versa. Thus I would recommend to have both.


The molecular biology bible
Any lab serious about molecular biology has this book. A lab staple for over 20 years the new updated version is even more comprehensive reflecting the continuing development of molecular biology. Most importantly the series still retains the historical significance and the background or reference material for many of the techniques. A must have!


An Essential Reference Book
A traditional work of reference in molecular biology, lately and completely updated. It has the wide insurance of almost all the molecular techniques of biology of core and often presents several various means of achieving the same goal. It is easy to reach the Web site and the protocols are clearly written. The Web site makes it easy to print out of the protocols. Moreover each protocol contains references to the primary education literature supporting its complaints. In the version of Internet of the protocol that the references are hotlinked with PubMed, simplifying the access to the fundamental references considerably. I recommend certainly this book to all the laboratories by using molecular biology. Take care to check the Web site!


a biology graduate student
Sambrook and Russell answer every question you could think of, and then some. This book is a goldmine of information, packed with protocols, but also filled with the extra information that transforms a simple set of instructions into an amazingly helpful how-to manual. The approach taken is that of an experimentor (ie. 'How to win the battle with RNase'--it really is a battle!), with hints and suggestions usually learned by watching an old pro. The information is well-organized, and very well illustrated to give a clear view of how an experiment is performed and especially the logic behind it. The manual also satisfies the insatiable curiosity of a scientist rather than a technician: how do pharmacological agents work? What are their structures? And the troubleshooting sections provide an invaluable resource. All in all, Sambrook and Russell have created an essential weapon in any scientist's artillery.


The bible of molecular cloning-updated
Molecular cloning has been a lab staple for years. Now reprinted so you can update the old lab copy worn out by years of student use! Its a must have for any lab serious about molecular biology. Its also useful for student training. Many times there are simple explanations for the lab techniques we have adopted as dogma, but are unsure why. Molecular cloning has the answers and is a great resource. I highly recommend this book for its depth and breadth of protocols and guidance in the complicated realm of cloning!


The holy scripture of molecular biology
This book is a priceless resource of value for any serious expert of molecular biology. The contents are detailed and complete. Strongly recommended source of reference. All the credit rating to the authors as regards a complete revision the latter will what is undoubtedly the scripture indicated of molecular biology. The Web site gives you the access to all the printable protocols of the book and is perfect for the attached student of graduate of laboratory.


Read it, there is another volume
You should read the improved version. This version misses things but also explains the alot. If you practise molecular biology you should almost keep this book


Long but Knowledgeable
In the laboratory where I work we have this volume. We were necessary to read certain parts if it. I loved how the books explained the complicated parts of molecular cloning. The bad part the books are very a long time.


A MUST-HAVE for all basic science researchers
This set of three manuals is invaluable in the lab. Complete with almost every technique commonly used, and full useful tables and other data sets that save the user a lot of time. One key point though, is that a new edition is long overdue.



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